Immunophenotypic Pattern of Eosinophilia by Flow Cytometry

Yusong Yang, David Gay, Zarina Arutyunova, Donghong Cai

Abstract


Eosinophilia is commonly seen in variety of conditions, including infections, hypersensitivities, autoimmune disorders, idiopathic hypereosinophilic syndrome and certain neoplasms. In the latter, eosinophilia may present as a secondary (benign) component (Hodgkin lymphoma, T-cell lymphoma, acute leukemia or systemic mastocytosis) or as myeloproliferative neoplasm (MPN) characterized by clonal eosinophilic expansion. MPN with eosinophilia includes chronic eosinophilic leukemia (CEL) and MPN with rearrangement of PDGFRA, PDGFRB or FGFR1. Identification of eosinophilia by flow cytometry (FC) may be difficult due to (1) lack of eosinophil-specific immunophenotypic markers, (2) overlap of phenotypic features of eosinophils with other populations and (3) low forward scatter leading to confusion with cellular debris. Based on immunophenotypic analysis of 15 cases, here we present the FC pattern of eosinophilia easily recognizable by routine flow cytometric analysis in a clinical laboratory. Identification of eosinophilia by FC with follow-up reflex cytogenetic/FISH and/or PCR testing may be helpful to differentiate between reactive conditions and malignant eosinophilic disorders when clinically indicated.  


Keywords


flow cytometry, eosinophilia, immunophenotypic pattern, granulocytes, neoplastic promyelocytes, basophils, MDS

References


Metcalf D, Begley CG, Nicola NA, et al. Quantitative responsiveness of murine hemopoietic populations in vitro and in vivo to recombinant multi-CSF (IL-3). Exp Hematol. 1987;15:288-295.

Metcalf D, Burgess AW, Johnson GR, et al. In vitro actions on hemopoietic cells of recombinant murine GM-CSF purified after production in Escherichia coli: comparison with purified native GM-CSF. J Cell Physiol. 1986;128:421-431.

Yamaguchi Y, Suda T, Shiozaki H, et al. Role of IL-5 in IL-2-induced eosinophilia. In vivo and in vitro expression of IL-5 mRNA by IL-2. J Immunol. 1990;145:873-877.

Hogan SP. Recent advances in eosinophil biology. Int Arch Allergy Immunol. 2007;143(Suppl 1):3-14.

Renauld JC. New insights into the role of cytokines in asthma. J Clin Pathol. 2001;54:577-589.

Zimmermann N, Hershey GK, Foster PS, et al. Chemokines in asthma: cooperative interaction between chemokines and IL-13. J Allergy Clin Immunol. 2003;111:227-242.

Lintomen L, Franchi G, Nowill A, et al. Human eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: lack of interaction with nitric oxide. BMC Pulm Med. 2008;8:13-19.


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